The effect of endurance training with and without vitamin E on expression of p53 and PTEN tumor suppressing genes in prostate glands of male rats
نویسندگان
چکیده
The aim of this study was to investigate the effect of endurance training with and without vitamin E on the expression of p53 and Phosphatase and tension homolog (PTEN) tumor suppressor genes of prostate glands in male rats. For this purpose, 50 Sprague-Dawley male rats were randomly assigned into 5 groups: (1) control group (CON, n = 10), (2) sham (S, n = 10), (3) endurance training (ET, n = 10), (4) endurance training + vitamin E (ET + VE, n = 10), (5) vitamin E (VE, n = 10). Endurance training protocol was implemented for 6 weeks, 6 days per week, in accordance with the overload principle. To measure expression changes of p53 and PTEN genes in rats' prostate, real-time PCR method was used and HPLC method was used to measure vitamin E in this tissue. After 6 weeks of taking vitamin E, its level in all groups, except for group VE (p < 0.000) did not significantly increase. After implementing training protocol, p53 expression reduced significantly in ET group (p < 0.026). Vitamin E supplementation along with endurance training did not cause any significant change either p53 or PTEN (respectively; p < 0.2, p < 0.11). Instead, vitamin E supplementation without endurance training caused significant increase in PTEN, but did not cause any significant changes in p53 (respectively; p < 0.016, p < 0.15). These results indicate that endurance training reduces p53 and PTEN tumor suppressing genes expression, and taking vitamin E supplement could increase expression of these genes in some extent.
منابع مشابه
The effect of short endurance training on the expression level of PINK-1, Parkin and PGC-1α in the heart of nicotine-sensitized rats
Background: Nicotine alters the expression of various genes in the heart. PINK-1(PTEN-induced kinase1) is the major regulator of cellular mitophagy. Moreover, Parkin is a protein that plays a key role in the process of ubiquitination. Also, PGC-1ὰ (peroxisome proliferator-activated receptor gamma coactivator 1-alpha) is the main regulator of mitochondrial biogenesis. On the other hand, exercise...
متن کاملThe Effect of Endurance Training on Expression of Autophagy Genes (Beclin-1, ULK-1) in the Heart Tissue of Male Rats with Experimental Diabetes
Objective: This study aimed to investigate the effect of six weeks of endurance training on the expression of autophagy-related genes (Beclin-1, ULK-1) in the heart tissue (Myocardium) of rats with diabetes. Materials and Methods: Twenty male wistar rats (weight: 204±11.3g), (age= 8 weeks) were divided into four groups including: 1) diabetic type1 training, group 2) diabetic type1 control, gro...
متن کاملThe effect of endurance training[a1] on Change of MYH7 and MYH7b genes expression and left ventricular structural changes of wistar male rats
Abstract Background; the MYH7 and MYH7b genes have important role on heart function on other hand endurance training is a challenge for heart function, therefore the aim this study was to study The effect of endurance training[a2] on Change of MYH7and MYH7b genes expression and left ventricular structural changes of wistar male rats. Material and Methods; For this purpose, 14 rats (113±20...
متن کاملThe effect of Origanum vulgare extract supplementation with and without exercise training on p53 and cytochrome c gene expression in myocardium of male rats
Background and Objective: Apoptosis plays a key role in the pathogenesis of a variety of cardiovascular diseases, especially cardiac failure. Therefore, the purpose of this study was investigating the effect of Origanum vulgare extract supplementation with and without exercise training on p53 and cytochrome c gene expression in myocardium of male rats. Materials and Methods: Nineteen three-...
متن کاملEffect of Spinal Nerve Ligation after Endurance Training on the Gene Expression of MST1 and MAFbx in Plantaris Muscle of Male Wistar Rats
Background and purpose: Reduction of muscle mass occurs in some models of muscle atrophy during mechanical unloading status and MST1 and MAFbx genes are believed to have a role. In the present study, the effect of reduced physical activity in the form of spinal nerve ligation (SNL) after a period of endurance training (ET) on the expression of MST1 and MAFbx genes were examined in the rat plant...
متن کامل